Domains of expertise : Bioanalysis using Mass Spectrometry |
FIVE MASS-SPECTROMETER APPARATUS (SCIEX, Thermo-electron)
LC-Mass Mass spectrometry (in tandem) : SCIEX API's 4000, THERMO Ultra, access to Ion Trap.
The team is experienced with ion-paired volatile mobile phases and in coupling techniques LC-Mass.
Compound |
Validated limit of quantitation |
nicotine ..................................................................
morphine ...............................................................
cocaine ..................................................................
melatonin ..............................................................
selegilin .................................................................
various polypeptides ...............................................
numerous antibiotics .............................................. |
250 pg/mL of plasma
1 pg/mL of plasma & e.c.fluid
500 pg/mL of plasma
1 pg/mL of plasma
100 pg/mL of plasma
20 - 50 pg/mL of plasma
100 pg/mL of plasma |
Matching of Triple-quad with Ion-Trap equipment’s provides powerful methodologies for structural metabolism involvements.
Use of multiprobe II for spe automatisation.
 Deuterated internal standards
For all the compounds studied at BIOTEC CENTRE, a team of chemists synthesises deuterated compounds as internal standards. This team is also highly involved in synthesis of haptens for immunochemistry as well as synthesis of potential metabolites.
Contact : Norbert BROMET, Maguy BROMET-PETIT, Ph.D. and Jordan Gonçalves |
BIOANALYSIS using UV, ECD & others |
LC - coupled to UV, Fluo, Radio, ECD
All software’s are GLP compliant with audit trail (EZ chrom-Elite from VWR, Flo-One from Packard/Perkin Elmer)
FLO-One radioactive detection
A radio-chromatogram example
FLO-One radioactive detection
Contact : Maguy BROMET-PETIT, Ph.D.
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France - Phone : +(33) 2 38 76 20 60 - Fax : +(33) 2 38 76 20 59 - Contact E-mail