IN VITRO DRUG METABOLISM
Biotec Centre has a strong background in metabolism pathway and biotransformation of pharmaceutical and chemical compounds.
CYP INVOLVEMENT
CYP INHIBITION
CYP’s involved in the formation of metabolites are identified. Prediction of potential drug-drug interaction or inter individual variability.
CYP INDUCTION
Cultured hepatocytes.
PROTEIN BINDING
Plasma of animals and specific human proteins.
MATERIALS AND METHODS for in VITRO/VIVO
Use of radiolabelled compounds. Extraction, separation, purification by LLE and/or SPE techniques.
Chromatographic methods, Flo-One radio-detection.
Structural determination by LC-MS (triple-quad &ion trap), NMR.
FEED BACK to BIOANALYSIS
Since these studies are carried out very early, active metabolites are included as well as the parent compound in the plasma analytical method. |
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IN VIVO DRUG METABOLISM
On different animal species and healthy volunteers: plasma, urine, faeces media using radio labelled isotopes.
From in and out patients.
Mass balance including pulmonary excretion (rat, dog and human).
On plasma, urine, faeces from animal during toxicokinetic and toxicology studies.
Residue studies in cattle, cow milk, swine, poultry.
On tissue homogenates using PRECELLYS®.
On vegetal during growth wheat colza, corn, etc…
Others: pollen, beeswax, honey and bees.
Water and soil residues for chemicals and phytocompounds.
Distribution studies in plant by QLRG using radiolabelled compounds.
Distribution studies by QLRG in fish, pigeon, etc…
MULTISPECIES COMPARISON
For pharmaceutical compounds as well as chemical compounds, metabolite profiling, disappearance time (DT50) for degradation products.
METABOLITE AND DEGRADATION PRODUCT
Chemical synthesis of metabolites, deuterated internal standards.
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